Mouse CD19 Protein, Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
CD9-M5257-100ug
$410.00
CD9-M5257-1mg (500ug X 2)
$2880.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    CD19, B4, CVID3, MGC12802

  • Source

    Mouse CD19 Protein, Fc Tag (CD9-M5257) is expressed from human 293 cells (HEK293). It contains AA Arg 19 - Gly 287 (Accession # P25918).

    Predicted N-terminus: Arg 19

    Request for sequence
  • Molecular Characterization

    CD19 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 56.0 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD19 SDS-PAGE

    Mouse CD19 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD19 SEC-MALS

    The purity of Mouse CD19 Protein, Fc Tag (Cat. No. CD9-M5257) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD19 ELISA

    Immobilized Rat/IgG2a,Kappa Anti-CD19 Antibody at 0.5 μg/mL (100 μL/well) can bind Mouse CD19 Protein, Fc Tag (Cat. No. CD9-M5257) with a linear range of 1-39 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     CD19 SPR

    Rat/IgG2a,Kappa Anti-CD19 Antibody immobilized on CM5 Chip can bind Mouse CD19 Protein, Fc Tag (Cat. No. CD9-M5257) with an affinity constant of 94.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

Ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase-3), encoded by the ENTPD3 gene and also known as CD39L3, is an integral membrane protein with an extracellular active site (1). Recombinant human NTPDase-3 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-3 hydrolyzes the beta - and gamma -phosphate residues of nucleotides, Has a threefold preference for the hydrolysis of ATP over ADP. Through its hydrolysis of extracellular nucleotid
es, NTPDase-3 plays a role in the regulation of purinergic signaling. The enzyme is expressed at its highest levels in brain, pancreas, spleen and prostate tissues.

Recent Advances

 
Drug Development Progress
  • English Name:

    B-lymphocyte antigen CD19

  • Category:

  • Approved Drugs:

    12 Details

  • Drugs in Clinical Trials:

    310 Details

  • Highest Development Stage:

    NDA/BLA

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