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Biotinylated Human CD1d&B2M Monomer Protein (Peptide free, MALS verified)

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CDM-H82E9-25ug
$120.00
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CDM-H82E9-200ug
$680.00
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CDM-H82E9
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Synonyms

CD1d & B2M

Source

Biotinylated Human CD1d&B2M Monomer Protein (CDM-H82E9) is expressed from human 293 cells (HEK293). It contains AA Glu 20 - Ser 301 (CD1d) & Ile 21 - Met 119 (B2M) (Accession # P15813 (CD1d) & P61769 (B2M)).

Predicted N-terminus: Glu 20 & Ile 21

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 39.0 kDa and 15.7 kDa. The protein migrates as 50-55 kDa and 17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Endotoxin

Less than 0.1 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

CD1d is a membrane protein non-covalently bonded to β2-microglobulin (β2m) and shows high homology between human and mice. CD1d can present α-galactosylceramide (α-GalCer), a glycolipid extracted and isolated from the marine sponge, and this complex can activate human and murine CD1d restricted NKT cells.