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Product Details
Synonyms
TNFRSF8, CD30, D1S166E, Ki-1
Source
Alexa Fluor 488-Labeled Human CD30 Protein, His Tag (CD0-HA2H7) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 40.4 kDa. The protein migrates as 55-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF488 to protein molar ratio is 0.5-2.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Alexa Fluor 488-Labeled Human CD30 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Alexa Fluor 488-Labeled Human CD30 Protein, His Tag (Cat. No. CD0-HA2H7) was greater than 90% as determined by SEC-HPLC.
Bioactivity-FACS
2e5 of anti-CD30 CAR-293 cells were stained with 100 μL of 3 μg/mL of Alexa Fluor 488-Labeled Human CD30 Protein, His Tag (Cat. No. CD0-HA2H7) and negative control protein respectively, washed and analyzed with FACS (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 8
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
22 Details
- Highest Development Stage：
Approved
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