Human CD30 Ligand / TNFSF8 Protein, Mouse IgG2a Fc Tag, low endotoxin

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CDL-H525b-100ug
$395.00
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CDL-H525b-1mg (250ug X 4)
$2,615.00
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Synonyms

CD30-L, CD153, TNFSF8, CD30L, CD30LG, CD153 antigen, CD30 antigen ligand, CD30 Ligand

Source

Human CD30 Ligand Protein, Mouse IgG2a Fc Tag (CDL-H525b) is expressed from human 293 cells (HEK293). It contains AA Gln 63 - Asp 234 (Accession # P32971-1).

Predicted N-terminus: Glu

Molecular Characterization

This protein carries a mouse IgG2a Fc tag at the N-terminus.

The protein has a calculated MW of 46.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

CD30 ligand (CD30L) and CD30 are interacting cellsurface glycoproteins that are members of the tumour necrosis factor (TNF) and tumour necrosis factor receptor
(TNFR) superfamilies. In humans, CD30L is expressed at high levels on activated T cells. There is conflicting evidence with regard to cell surface expression of CD30L on primary human B cells. An initial report of constitutive CD30L expression on peripheral blood B cells33 has not been substantiated.