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Product Details
Synonyms
CD38, T10, cADPr hydrolase 1
Source
Human CD38, His Tag (CD8-H5224) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # NP_001766).
Predicted N-terminus: Val 43
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.7 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.2 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM MES, 100 mM NaCl, pH6.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD38, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD38, His Tag (Cat. No. CD8-H5224) is more than 95% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD38, His Tag (Cat. No. CD8-H5224) at 5 μg/mL (100 μL/well) can bind Anti-CD38 MAb, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-CD38 mAb (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CD38, His Tag (Cat. No. CD8-H5224) with an affinity constant of 4.98 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of Anti-CD38 CAR-293 cells were stained with 100 μL of 10 μg/mL of Human CD38 Protein, His Tag (MALS verified)(Cat. No. CD8-H5224) and negative control protein respectively, washed and then followed by His Antibody, PE and analyzed with FACS (Routinely tested).
Protocol
Customer Reviews Writing Reviews
1***02019-10-11
LikesI bought a lot of products. This protein has high purity and good bioactivity. Whether it is for ELISA or functional assay, all went smoothly. The price is favorable and the service is also good. I support and recommend ACRO! Go ACRO!
Background
Recent Advances
Drug Development Progress- English Name：
Lymphocyte differentiation antigen CD38
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
47 Details
- Highest Development Stage：
Approved
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