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Product Details
Synonyms
CD38, T10, cADPr hydrolase 1
Source
Biotinylated Human CD38, Avitag,His Tag (CD8-H82E7) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # NP_001766.2).
Predicted N-terminus: Val 43
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 32.5 kDa. The protein migrates as 40-48 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human CD38, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human CD38, Avitag,His Tag (Cat. No. CD8-H82E7) is more than 85% and the molecular weight of this protein is around 35-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Anti-CD38 MAb, Human IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD38, Avitag,His Tag (Cat. No. CD8-H82E7) with a linear range of 0.08-1.28 μg/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of Anti-CD38 CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human CD38 Protein, Avitag,His Tag (Cat. No. CD8-H82E7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Y***u2023-03-31
LikesThis product can be used at < 0.4 ug/ml as a detection agent to quantify anti CD38 antibodies in a binding assay. Anti CD38 antibodies in either bovine serum albumin based sample diluent or murine serum can be detected by this production without a problem. Specific signals from the subsequent streptavidin conjugated enzyme reaction were very strong and very low noise from the negative control antibody group.
Background
Recent Advances
Drug Development Progress- English Name：
Lymphocyte differentiation antigen CD38
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
47 Details
- Highest Development Stage：
Approved
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