Human CD39L1 / ENTPD2 Protein, His Tag (active enzyme, MALS verified)

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Cat. No. / Size
Price
Qty
CD1-H53H3-20ug
$360.00
CD1-H53H3-100ug
$1010.00
CD1-H53H3-500ug
$3605.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD39L1, NTPDase-2

  • Source

    Human CD39L1 Protein, His Tag (CD1-H53H3) is expressed from CHO cells. It contains AA Thr 29 - Asp 460 (Accession # Q9Y5L3-1).

    Predicted N-terminus: Thr 29

    Request for sequence
  • Molecular Characterization

    CD39L1/ENTPD2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 49.0 kDa. The protein migrates as 36-37 kDa and 57-63 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD39L1/ENTPD2 SDS-PAGE

    Human CD39L1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    CD39L1/ENTPD2 SEC-MALS

    The purity of Human CD39L1 Protein, His Tag (Cat. No. CD1-H53H3) is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

    Report
  • Bioactivity

    Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 5,000 pmol/min/μg (QC tested).

    Protocol

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Background

CD39L1, also known as ENTPD2 and NTPDase2, is an ectonucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells . CD39L1 is a Ca2+ and Mg2+ dependent enzyme that hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP. CD39L1 plays a role in regulating thrombosis and inflammation . It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation.

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