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Product Details
Synonyms
CD3E & CD3D, CD3 delta & CD3 epsilon
Source
Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (CDD-HA2W5) is produced via conjugation of AF488 to Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free with a new generation site-specific technology under Star Staining labeling platform. Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
Predicted N-terminus: Asp 23 & Phe 22
Molecular Characterization
Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD3E and CD3D. Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Protein Ratio
The AF488 to protein molar ratio is 0.9-1.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.1-8 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
Bioactivity-Flow Cytometry
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor™ 488-Labeled Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag & Tag Free (Cat. No. CDD-HA2W5) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor™ 488-Labeled Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag & Tag Free (Cat. No. CDD-HA2W5) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
Bioactivity-Stability
Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W5) is stable at 25℃ for 50 hours, equivalent to store at -70 ℃ for 2 years and freezing and thawing 3 times without performance reduction.
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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