Save Product
Product Details
Synonyms
CD3 epsilon & CD3 gamma, CD3E & CD3G
Source
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDG-H52W9) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).
Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) is more than 95% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) at 2 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 0.4-13 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a)) with an affinity constant of 39.6 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Human CD3 antibody (UCHT1) with an affinity constant of 1.59 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Bispecific T-cell Engager (CD3 X BCMA) with an affinity constant of 1.13 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
