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Product Details
Synonyms
CD58, LFA3, Ag3
Source
Human CD58, Fc Tag (LF3-H5256) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Arg 215 (Accession # Q9BRW0).
Predicted N-terminus: Phe 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.1 kDa. The protein migrates as 60-70 kDa under reducing (R) condition, and 150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to different glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD58, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human CD2, His Tag (Cat. No. CD2-H5226) at 2 μg/mL (100 μL/well) can bind Human CD58, Fc Tag (Cat. No. LF3-H5256) with a linear range of 10-78 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded bind Human CD58, Fc Tag (Cat. No. LF3-H5256) on Protein A Biosensor, can bind Human CD2, His Tag (Cat. No. CD2-H5226) with an affinity constant of 0.49 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded bind Human CD58, Fc Tag (Cat. No. LF3-H5256) on AHC Biosensor, can bind Human CD2, His Tag (Cat. No. CD2-H5226) with an affinity constant of 0.57 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Lymphocyte function-associated antigen 3
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Clinical
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