Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, Fc Tag&Fc Tag, low endotoxin (MALS verified)

Synonyms

CD8 alpha & beta, CD8A & CD8B

Source

Human CD8A&CD8B Heterodimer Protein, Fc Tag&Fc Tag (CDA-H5253) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 & Leu 22 - Pro 170 (Accession # P01732-1 & P10966-1).

Predicted N-terminus: Ser 22 & Leu 22

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Human CD8A&CD8B Heterodimer Protein, Fc Tag&Fc Tag, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 44.1 kDa (CD8 alpha) and 43.0 kDa (CD8 beta). Subunit CD8 alpha is fused with a human IgG1 Fc fragment at the C-terminus and subunit CD8 beta is fused with a human IgG1 Fc fragment at the C-terminus. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterodimer.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process