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Human CD8 beta Protein, His Tag (MALS verified)

Related Products: Cell line CJUR-STP272
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CD8-H52H5-100ug
$450.00
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CD8-H52H5-1mg (500ug X 2)
$2,980.00
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CD8-H52H5
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

CD8B, CD8B1, LEU2, LY3, LYT3, P37

Source

Human CD8 beta Protein, His Tag (CD8-H52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 22 - Pro 170 (Accession # P10966-1).

Predicted N-terminus: Leu 22

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 18.7 kDa. The protein migrates as 27-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a dimer.

Endotoxin

Less than 0.05 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

CD8B, also named P37 and LEU2, serves as the beta subunit of CD8 molecule with one Ig-like V-type domain. It mainly expresses on cytotoxic T lymphocytes and forms a heterodimer with CD8α. As an essential co-receptor, it cooperates with TCR to recognize MHC class I-restricted antigens on APCs and stabilizes immune cell interactions. The CD8B gene generates multiple spliced variants encoding membrane-bound and secreted isoforms, and participates in T cell activation and cytotoxic immune responses.