Human CD99 / MIC2 Protein, Llama IgG2b Fc Tag, low Endotoxin (MALS verified)

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CD9-H5254-100ug
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CD9-H5254
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Synonyms

CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X, 12E7

Source

Human CD99 Protein, Llama IgG2b Fc Tag (CD9-H5254) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 122 (Accession # AAH02584).

Predicted N-terminus: Asp 23

Molecular Characterization

This protein carries a llama IgG2b Fc tag at the C-terminus.

The protein has a calculated MW of 37.9 kDa. The protein migrates as 55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A.