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Product Details
Synonyms
Coagulation factor XI, FXI, PTA, F11
Source
Human Coagulation factor XI, His Tag (FXI-H52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Val 625 (Accession # P03951-1).
Predicted N-terminus: Glu 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 69.9 kDa. The protein migrates as 35 kDa and 50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Coagulation factor XI, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Coagulation factor XI, His Tag (Cat. No. FXI-H52H5) at 1 μg/mL (100 μL/well) can bind Abelacimab with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Immobilized Abelacimab at 1 μg/mL (100 μL/well) can bind Human Coagulation factor XI, His Tag (Cat. No. FXI-H52H5) with a linear range of 2-31 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-Factor XI Antibody captured on Protein A Chip can bind Human Coagulation factor XI, His Tag (Cat. No. FXI-H52H5) with an affinity constant of 15.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >300 pmol/min/µg (QC tested)
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Coagulation factor XI
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
31 Details
- Highest Development Stage：
Approved
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