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Product Details
Synonyms
DKK1, SK
Source
Human Dkk-1, Fc Tag (DK1-H5258) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1).
Predicted N-terminus: Thr 32
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.4 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Dkk-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Dkk-1, Fc Tag (Cat. No. DK1-H5258) is more than 90% and the molecular weight of this protein is around 118-145 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Dkk-1, Fc Tag (Cat. No. DK1-H5258) at 2 μg/mL (100 μL/well) can bind Human Kremen-2, His Tag (Cat. No. KR2-H52H3) with a linear range of 5-78 ng/mL (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human Dkk-1 Protein, Fc Tag (MALS verified) (Cat. No. DK1-H5258) inhibits Wnt induced TCF reporter activity in HEK293 cells. Human Dkk-1 inhibits a constant dose of 0.03 µg/mL of Wnt surrogate. The EC50 for this effect is 0.051 µg/mL.
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Dickkopf-related protein 1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 2 Clinical
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