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Product Details
Synonyms
TNFRSF25, DR3, APO3, DDR3, TNFRSF12, WSL, WSL1
Source
Biotinylated Human DR3 Protein, His,Avitag (DR3-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 199 (Accession # Q93038-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22.6 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human DR3 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human DR3 Protein, His,Avitag (Cat. No. DR3-H82E3) is more than 90% and the molecular weight of this protein is around 25-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DR3 Protein, His,Avitag (Cat. No. DR3-H82E3) with a linear range of 0.02-2.5 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Human DR3 Protein, His,Avitag (Cat. No. DR3-H82E3) captured on Biotin CAP-Series S Sensor Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 1.75 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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