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Product Details
Synonyms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Source
Mouse EGF R, His Tag (EGR-M5224) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 647 (Accession # Q01279-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 71.2 kDa. The protein migrates as 86-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Mouse EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse EGF R, His Tag (Cat. No. EGR-M5224) is more than 90% and the molecular weight of this protein is around 85-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse EGF, Fc Tag (Cat. No. EGF-M5265) at 2 μg/mL (100 μL/well) can bind Mouse EGF R, His Tag (Cat. No. EGR-M5224) with a linear range of 0.02-0.313 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Captured Mouse EGF R, His Tag (Cat. No. EGR-M5224) on CM5 Chip via anti-His antibody can bind Mouse EGF, Fc Tag (Cat. No. EGF-M5265) with an affinity constant of 5.44 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Epidermal growth factor receptor erbB1
- Category：
- Approved Drugs：
39 Details
- Drugs in Clinical Trials：
206 Details
- Highest Development Stage：
Approved
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