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Human ENPP-2 / Autotaxin Protein, His Tag (active enzyme)

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Cat. No. / Size
Price
Qty
EN2-H5241-50ug
$300.00
EN2-H5241-250ug
$660.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    ENPP-2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA

  • Source

    Human ENPP-2, His Tag (EN2-H5241) is expressed from human 293 cells (HEK293). It contains AA Asp 49 - Ile 863 (Accession # NP_001035181).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    ENPP-2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 94.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ENPP-2 SDS-PAGE

    Human ENPP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity

    Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >8,000 pmol/min/μg, as measured under the described conditions (QC tested).

    Protocol

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Background

Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.

Recent Advances

 
Drug Development Progress
  • English Name:

    Ectonucleotide pyrophosphatase/phosphodiesterase 2

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    9 Details

  • Highest Development Stage:

    Phase 2 Clinical

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