Human EpCAM / TROP1 Protein, Fc Tag

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Cat. No. / Size
Price
Qty
EPM-H5254-100ug
$310.00
EPM-H5254-1mg (500ug X 2)
$1950.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    EPCAM, TACSTD1, TROP1, CD326, DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1, 4, KSA, M4S1, MIC18, MK1

  • Source

    Human EpCAM, Fc Tag (EPM-H5254) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1).

    Predicted N-terminus: Gln 24

    Request for sequence
  • Molecular Characterization

    EpCAM Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 53.5 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.2 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    EpCAM SDS-PAGE

    Human EpCAM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     EpCAM ELISA

    Immobilized Human EpCAM, Fc Tag (Cat. No. EPM-H5254) at 1 μg/mL (100 μL/well) can bind Anti-EpCAM Antibody, Human IgG1 with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol

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Background

EpCAM is also known as CO171A, EGP, EGP40,GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy.
Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.

Recent Advances

 
Drug Development Progress
  • English Name:

    Epithelial cell adhesion molecule

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    11 Details

  • Highest Development Stage:

    Approved

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