Product Details
Synonyms
EPCAM, TACSTD1, TROP1, CD326, DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1, 4, KSA, M4S1, MIC18, MK1
Source
Biotinylated Rhesus macaque EpCAM / TROP1 Protein, His,Avitag (EPM-R82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # Q1WER1).
Predicted N-terminus: Gln 24
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 31 kDa. The protein migrates as 37-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Rhesus macaque EpCAM / TROP1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Biotinylated Rhesus macaque EpCAM / TROP1 Protein, His,Avitag (Cat. No. EPM-R82E3) is more than 90% and the molecular weight of this protein is around 28-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Biotinylated Rhesus macaque EpCAM / TROP1 Protein, His,Avitag (Cat. No. EPM-R82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-EpCAM Antibody, Human IgG1 with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
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Background
Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
Recent Advances
- English Name:
Epithelial cell adhesion molecule
- Category:
- Approved Drugs:
1 Details
- Drugs in Clinical Trials:
11 Details
- Highest Development Stage:
Approved
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