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Product Details
Synonyms
Ephrin-A3, EFL-2, EHK1-L, LERK-3, EFNA3
Source
Human Ephrin-A3, Fc Tag (EA3-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Ser 213 (Accession # AAI10407).
Predicted N-terminus: Gln 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.4 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 100 mM Glycine, 150 mM Nacl, 25 mM Arginine, 50 mM Tris, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Ephrin-A3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human EphA10, His Tag (Cat. No. EP0-H52H3) at 5 μg/mL (100 μL/well) can bind Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) at 5 μg/mL (100 μL/well) can bind Human EphA10, His Tag (Cat. No. EP0-H52H3) with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Ephrin-A3, Fc Tag (Cat. No. EA3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human EphA5, His Tag (Cat. No. EP5-H52H5) with an affinity constant of 0.576 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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