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Human ErbB3 / Her3 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
ER3-H5223-100ug
$310.00
ER3-H5223-1mg (500ug X 2)
$1950.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3

  • Source

    Human ErbB3, His Tag (ER3-H5223) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # P21860-1).

    Predicted N-terminus: Ser 20

    Request for sequence
  • Molecular Characterization

    ErbB3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 70.5 kDa. The protein migrates as 70-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ErbB3 SDS-PAGE

    Human ErbB3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    ErbB3 SEC-MALS

    The purity of Human ErbB3, His Tag (Cat. No. ER3-H5223) is more than 95% and the molecular weight of this protein is around 75-100 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     ErbB3 ELISA

    Immobilized Human ErbB3, His Tag (Cat. No. ER3-H5223) at 2 μg/mL (100 μL/well) can bind Human NRG1 Beta 1, Fc Tag, premium grade (Cat. No. NR1-H5268) with a linear range of 0.1-3 ng/mL (QC tested).

    Protocol

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Background

ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Recent Advances

 
Drug Development Progress
  • English Name:

    Receptor tyrosine-protein kinase erbB-3

  • Category:

  • Approved Drugs:

    2 Details

  • Drugs in Clinical Trials:

    31 Details

  • Highest Development Stage:

    Approved

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