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Product Details
Synonyms
Fc gamma RIIIB , CD16b (NA1), FCGR3B, CD16B, FCG3B, FCGR3, FCG3, IGFR3
Source
Biotinylated Human CD16b (NA1), His,Avitag (CDB-H82E4) is expressed from human HEK293 cells. It contains AA Gly 17 - Ser 200 (Accession # AAA35881.1). The NA1 form of the CD16b differ with the NA2 form of CD16b in AA36, 65, 82, and 106. The NA1 form carries R36, N65, D82, and V106, while the NA2 form carries S36, S65, N82, and I106.
Predicted N-terminus: Gly 17
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 24.6 kDa. The protein migrates as 38-48 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human CD16b (NA1), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) is more than 95% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) on Biotin CAP - Series S sensor Chip can bind Monoclonal Anti-Her2 Antibody, Human IgG1 (Cat. No. HE2-M598) with an affinity constant of 28.3 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Immobilized Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) on SA Chip, can bind Herceptin with an affinity constant of 3.31 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human CD16b (NA1), His,Avitag (Cat. No. CDB-H82E4) on SA Biosensor, can bind Rituximab with an affinity constant of 7.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
CD16B is also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. FCGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, FCG3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
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