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Product Details
Synonyms
FcRn, FCGRT & B2M
Source
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (FCM-H82W4) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)).
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 38 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.
Conjugate
Biotinylated
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CTLA-4 Antibody, Human IgG1 with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 0.369 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP - Series S sensor Chip can bind Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) with an affinity constant of 0.728 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP - Series S sensor Chip can bind Human IgG2 Fc, Tag Free (Cat. No. IG2-H5206) with an affinity constant of 0.113 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP - Series S sensor Chip can bind Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) with an affinity constant of 3.32 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP - Series S sensor Chip can bind Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205) with an affinity constant of 1.06 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) on SA Biosensor, can bind Herceptin with an affinity constant of 0.22 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) on SA Biosensor, can bind Human Serum Albumin, His Tag (Cat. No. HSA-H5220) with an affinity constant of 0.772 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
IgG receptor FcRn large subunit p51
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Approved
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