Recombinant Fibronectin fragment, Research GradeResearch use only

  • This product is designed for academic and scientific research institutions.
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Cat. No. / Size
Price
Qty
FIN-H5117-500ug
$100.00
ETA of in-stock products:2 business days

Product Details

  • Features and Advantages

    1. Native Conformation: Native sequences, tag-free and natural function.
    2. Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
    3. Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
    4. Lowest Endotoxin level (<0.01 EU/ug).
    5. Safety Assurance: Sterile filtration through 0.2 μm membrane.
    6. AOF: Animal origin-free raw materials throughout the production process.
  • Synonyms

    Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF

  • Source

    Recombinant Fibronectin fragment, Research Grade (FIN-H5117) is expressed from E. coli cells. It contains AA Pro 1361 - Ser 1637 & Ala 1812 - Thr 2107 (Accession # P02751-15).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Fibronectin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 62.6 kDa. The protein migrates as 60 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Supplied as 0.2 μm filtered solution in 12.5 mM Citric acid, pH6.2 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C for 12 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process
  • Quality Description

    ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
    ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.

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Performance Data

  • SDS-PAGE

    Fibronectin SDS-PAGE

    Recombinant Fibronectin fragment, Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    Fibronectin SEC-HPLC

    The purity of Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) was greater than 90% as determined by SEC-HPLC.

  • Bioactivity-SPR

     Fibronectin SPR

    Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) immobilized on CM5 Chip can bind Heparin Sulfate with an affinity constant of 0.24 μM as determined in a SPR assay (Biacore T200) (QC tested).

    Protocol
  •  Fibronectin SPR

    Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) immobilized on SA Chip can bind Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) with an affinity constant of 43.4 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol
  •  Fibronectin SPR

    Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) immobilized on SA Chip can bind Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) with an affinity constant of 0.823 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

Fibronectin (Fn) is a glycoprotein whose size ranges from 230 to 270 kDa and usually exists as a dimer, covalently linked by a pair of disulfide bonds at the C-termini. Each monomer consists of three repeating units: 12 Type I, 2 Type II, and 15–17 Type III domains which combined account for 90% of the FN sequence. The extracellular matrix (ECM) plays a key role as both structural scaffold and regulator of cell signal transduction in tissues. Fibronectin is one of the major ECM proteins in the trabecular meshwork (TM). It is found in the sheath material surrounding the elastin tendons that enter the TM from the ciliary muscle within the ciliary body. In times of ECM assembly and turnover, cells upregulate assembly of the ECM protein, FN. FN is assembled by cells into viscoelastic fibrils that can bind upward of 40 distinct growth factors and cytokines. These fibrils play a key role in assembling a provisional ECM during embryonic development and wound healing. Fibril assembly is also often upregulated during disease states, including cancer and fibrotic diseases.

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