Product Details
Synonyms
GAPDH, GAPD, CDABP0047, OK, SW-cl.12
Source
Human GAPDH, His Tag (GAH-H5145) is expressed from E. coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 36.9 kDa. The protein migrates as 37-38 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human GAPDH, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA

Immobilized Human GAPDH, His Tag (Cat. No. GAH-H5145) at 1 μg/mL (100 μL/well) can bind GAPDH Monoclonal Antibody, Mouse IgG with a linear range of 0.5-31 ng/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Glyceraldehyde-3-phosphate dehydrogenase liver
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
0 Details
- Highest Development Stage:
Preclinical
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