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Product Details
Synonyms
GPC3, OCI5, Glypican-3, GTR2-2, MXR7, DGSX, SDYS , SGB, SGBS, SGBS1
Source
Human Glypican 3 (S359F) Protein, His Tag (GP3-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1(S359F)).
Predicted N-terminus: Gln 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Glypican 3 (S359F) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human Glypican 3 (S359F) Protein, His Tag (Cat. No. GP3-H5223) at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPC3 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human Glypican 3 (S359F) Protein, His Tag (Cat. No. GP3-H5223) at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 Mab with a linear range of 0.4-3 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-Human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3 (S359F) Protein, His Tag (Cat. No. GP3-H5223) with an affinity constant of 1.96 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3 (S359F) Protein, His Tag (Cat. No. GP3-H5223) with an affinity constant of 3.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Glypican-3
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
57 Details
- Highest Development Stage：
Phase 2 Clinical
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