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Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein (MALS verified)

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HLP-H82Ee-25ug
$290.00
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HLP-H82Ee-200ug
$1,815.00
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HLP-H82Ee
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Product Details
Performance Data
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  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
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Synonyms

HLA-A*0201 & B2M & p53 (HMTEVVRRC)

Source

Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein (HLP-H82Ee) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & HMTEVVRRC peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & HMTEVVRRC).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

Human HLA-A*02:01&B2M&p53 (HMTEVVRRC) Tetramer Protein is assembled by biotinylated monomer and streptavidin.

Biotinylated HLA-A*02:01&B2M&p53 (HMTEVVRRC) Complex Protein is produced by co-expression of HLA-A*02:01 and B2M loaded with p53 peptide. Biotinylated HLA-A*02:01&B2M&p53 (HMTEVVRRC) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa and 13.3 kDa and 11.7 kDa. The protein migrates as 41-45 kDa, 12 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

TP53 has been recognized as a tumor suppressor. 50% of cancers carry a TP53 mutation while many others affect other pathway components. High-copy numbers of WT p53 peptide-MHC class I complexes were detected on tumor cells as compared to low copies on normal cells. The Human HLA-A*0201 p53 (HMTEVVRRC) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and HMTEVVRRC peptide of the p53.