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PE-Labeled Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer Protein

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HLE-HP2E3-25ug
$120.00
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HLE-HP2E3-200ug
$680.00
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Product Details
Performance Data
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  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
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Synonyms

HLA-A*0201 & B2M & PRAME (ALYVDSLFFL)

Source

PE-Labeled Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer Protein (HLE-HP2E3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & ALYVDSLFFL (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & ALYVDSLFFL).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

PE-Labeled Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Tetramer Protein is assembled by biotinylated monomer and PE-labeled streptavidin.

Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Complex Protein is produced by co-expression of HLA and B2M loaded with PRAME peptide. Biotinylated Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, 50-150 mM NaCl with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

PRAME (Preferentially Expressed Antigen in Melanoma) is a well-characterized member of the cancer/testis antigen family. Functioning as a nuclear receptor and transcriptional regulator, PRAME negatively modulates retinoic acid signaling, thereby regulating tumor cell differentiation and proliferation. It is aberrantly overexpressed in a variety of malignancies, especially cutaneous melanoma, and its upregulation is closely linked to aggressive tumor phenotypes. Given its tumor-specific expression and oncogenic role, PRAME has emerged as a promising diagnostic biomarker, prognostic indicator, and attractive therapeutic target for tumor immunotherapy.