Biotinylated Human HLA-A*24:02&B2M&EBV EBNA3A (RYSIFFDYM) Complex Protein (Monomer, MALS verified)

Synonyms

HLA-A*2402 & B2M & EBV EBNA3A (RYSIFFDYM)

Source

Biotinylated Human HLA-A*24:02&B2M&EBV EBNA3A (RYSIFFDYM) Complex Protein (HLA-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & RYSIFFDYM peptide (Accession # AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & RYSIFFDYM).

Predicted N-terminus: Gly 25 & Arg

Molecular Characterization

Biotinylated Human HLA-A*24:02&B2M&EBV EBNA3A (RYSIFFDYM) Complex Protein is produced by co-expression of HLA and B2M loaded with EBV EBNA3A peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 35.8 kDa and 14.0 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process