Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein (Monomer)

Synonyms

HLA-C*07:02:01 & B2M & RYR (RYRPGTVAL)

Source

Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein (HLR-H52W2) is expressed from human 293 cells (HEK293). It contains AA Cys 25 - Ile 308 (HLA-C*07:02:01) & Ile 21 - Met 119 (B2M) & RYRPGTVAL peptide (Accession # P10321 (HLA-C*07:02:01) & P61769 (B2M) & RYRPGTVAL).

Predicted N-terminus: Cys 25 & Arg

Molecular Characterization

Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein is produced by co-expression of HLA and B2M loaded with RYR peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 36.1 kDa and 13.8 kDa. The protein migrates as 42-45 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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