Human IDO1 Protein, His Tag (active enzyme, MALS verified)

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Cat. No. / Size
Price
Qty
ID1-H5143-100ug
$360.00
ID1-H5143-1mg
$2575.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IDO, IDO-1, INDO

  • Source

    Human IDO1 Protein, His Tag (ID1-H5143) is expressed from E. coli cells. It contains AA Ala 2 - Gly 403 (Accession # P14902-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    IDO1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 47.2 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IDO1 SDS-PAGE

    Human IDO1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IDO1 SEC-MALS

    The purity of Human IDO1 Protein, His Tag (Cat. No. ID1-H5143) is more than 85% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

    Report
  • Bioactivity

    Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is >400 pmol/min/μg (QC tested).

    Protocol

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Background

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Recent Advances

 
Drug Development Progress
  • English Name:

    Indoleamine 2,3-dioxygenase

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    12 Details

  • Highest Development Stage:

    Phase 3 Clinical

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