Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag™ (MALS & SPR verified)

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IF1-H82E3-25ug
$360.00
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IF1-H82E3-200ug
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IF1-H82E3
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Synonyms

IFNAR1, IFNAR, CRF2-1, IFN-R-1, IFNAR-1

Source

Biotinylated Human IFN-alpha / beta R1 Protein, His,Avitag (IF1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Lys 436 (Accession # P17181-1).

Predicted N-terminus: Lys 28

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 50.7 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Interferon alpha/beta receptor 1 ( IFR1) is also known as Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1 (CRF2-1), Type I interferon receptor 1, IFR, which belongs to the type II cytokine receptor family. IFR1 /IFR contains four fibronectin type-III domains. IFR1 associates with IFR2 to form the type I interferon receptor. IFR1 is receptor for interferons alpha and beta. IFR1 can transduce IFNB signals without the help of IFR2, and not activating the Jak-STAT pathway.