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Product Details
Synonyms
IL-12 R beta 1, IL12RB1, CD212, IL12R, IL12RB, IL-12RB1
Source
Biotinylated Human IL-12 R beta 1, Fc,Avitag (ILB-H82F7) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 540 (Accession # P42701-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 85.4 kDa. The protein migrates as 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IL-12 R beta 1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human IL-12B, His Tag (Cat. No. NK2-H52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) with a linear range of 10-78 ng/mL (QC tested).
Protocol
Immobilized Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) with a linear range of 10-78 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) on SA Biosensor, can bind ActiveMax® Human IL-12 ,Tag Free (Cat. No. IL2-H5210) with an affinity constant of 1.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-12 receptor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
9 Details
- Highest Development Stage：
Phase 2 Clinical
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