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Product Details
Synonyms
IL3R, IL3RA, IL-3Ra, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, CD123 antigen, CD123, hIL3Ra, hIL-3Ra, MGC34174, IL-3 R alpha
Source
Biotinylated Human IL-3 R alpha Protein, His,Avitag, premium grade (ILA-H82H5) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305 (Accession # P26951-1).
Predicted N-terminus: Thr 19
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.7 kDa. The protein migrates as 57 kDa±3 kDa under reducing (R) condition, and 54 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Negative
Mycoplasma
Negative
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human IL-3 R alpha Protein, His,Avitag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IL-3 R alpha Protein, His,Avitag, premium grade (Cat. No. ILA-H82H5) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-3 R alpha Protein, His,Avitag, premium grade (Cat. No. ILA-H82H5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-IL-3 R alpha Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Human IL-3 R alpha Protein, His,Avitag, premium grade (Cat. No. ILA-H82H5) immobilized on SA Chip can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with an affinity constant of 0.327 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Y***a2022-05-10
LikesWe received the product at a reasonably fast speed. We used this product in ELISA. The product is robust and of good quality. The characterization information was accurate, reliable, and valuable for further calculations.- E***z2022-04-22 LikesAfter struggling to express large quantities of this protein in-house, we ordered this product and used it as the bait in our antibody discovery pipeline and found to to be extremely high quality. We used this product in ELISA as well as BLI, and generated reproducible results. In addition, we found the batch biotinylation information to be accurate which was helpful for calculating inputs for experiments.
- R***r2021-10-28 LikesWe ordered this biotinylated recombinant CD123 protein from Acro while it was still under development. We were utilizing it to test CAR binding via flow cytometry. We contacted Acro and they were very helpful in being able to expedite the production of it for us to use. Since receiving it, we have not had any issues with it; it works beautifully and consistently on flow with very clean binding to streptavidin. The only reason I deducted a star from technical services was a slight lack of communication as far as when the product would be ready; it was still ready in a timely manner, but slightly behind schedule. However, they were very accommodating and helpful in getting us the protein quickly.
Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-3 receptor subunit alpha
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
40 Details
- Highest Development Stage：
Approved
Contact Us
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[Asia & Pacific]: +86 400-682-2521
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