Cynomolgus IL-23 alpha & IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)

Synonyms

IL-23 alpha & IL-12 beta

Source

Cynomolgus IL23A & IL12B Heterodimer Protein, His Tag&Tag Free (ILB-C52W3) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Pro 189 & Ile 23 - Ser 328 (Accession # G7PIH8 & G7P6S2).

Predicted N-terminus: Val 22 & Ile 23

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Cynomolgus IL23A & IL12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 20.3 kDa (IL23A) and 34.8 kDa (IL12B). Subunit IL-23A is fused with a polyhistidine tag at the C-terminus and subunit IL-12B contains no tag. The protein migrates as 22-25 kDa,38-39 kDa and 41-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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