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Product Details
Synonyms
Integrin alpha L beta 2, ITGAL&ITGB2
Source
Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free (IT2-H53W3) is expressed from CHO cells. It contains AA Tyr 26 - Met 1089 (ITGAL) & Gln 23 - Asn 700 (ITGB2) (Accession # P20701-1 (ITGAL) & P05107-1 (ITGB2) (H354Q)).
Predicted N-terminus: Tyr 26 (ITGAL) & Gln 23 (ITGB2)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAL and ITGB2, has a calculated MW of 124.1 kDa (ITGAL) & 80.2 kDa (ITGB2). Subunit ITGAL is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus. The protein migrates as 140-150 kDa (ITGAL) and 90-95 kDa (ITGB2) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H53W3) at 10 μg/mL (100 μL/well) can bind Human ICAM-1, Fc Tag (Cat. No. IC1-H5250) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Human ICAM-1, Fc Tag (Cat. No. IC1-H5250) at 10 μg/mL (100 μL/well) can bind Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H53W3) with a linear range of 0.156-5 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha-L/beta-2 (LFA-1)
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Approved
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