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Product Details
Synonyms
Integrin alpha V beta 6, ITGAV&ITGB6
Source
Biotinylated Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (IT6-C82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 993 & Gly 22 - Pro 709 (Accession # A0A2K5WCD3-1 & A0A2K5TZ36-1).
Predicted N-terminus: Phe 31 & Gly 22
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Other Tags and Version Biotin & Other Labeled Version
Biotinylated Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 114.8 kDa (ITGAV) & 79.9 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGAV) and 80-95 kDa (ITGB6) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-C82E3) with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Immobilized Fibronectin fragment, premium grade (Cat. No. FIN-H5113) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-C82E3) with a linear range of 2-78 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha-V/beta-6
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
6 Details
- Highest Development Stage：
Phase 2 Clinical
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