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Product Details
Synonyms
Legumain
Source
Mouse Legumain Protein, His Tag (LEN-M52H3) is expressed from human 293 cells (HEK293). It contains AA Val 18 - Tyr 435 (Accession # O89017-1).
Predicted N-terminus: Val 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.4 kDa. The protein migrates as 43-48 kDa and 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM Tris, 20 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Mouse Legumain Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Mouse Legumain Protein, His Tag (Cat. No. LEN-M52H3) was greater than 90% as determined by SEC-HPLC.
Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >350 pmol/min/μg (QC tested).
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