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Product Details
Synonyms
LILRB4, ILT3, LIR5, CD85K, HM18
Source
Cynomolgus LILRB4, His Tag (CDK-C5227) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # XP_015297198.1).
Predicted N-terminus: Gln 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.0 kDa. The protein migrates as 34-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus LILRB4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Cynomolgus LILRB4, His Tag (Cat. No. CDK-C5227) is more than 85% and the molecular weight of this protein is around 30-42 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Cynomolgus LILRB4, His Tag (Cat. No. CDK-C5227) at 2 μg/mL (100 μL/well) can bind Human ANGPTL7, His Tag with a linear range of 8-125 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Cynomolgus LILRB4, His Tag (Cat. No. CDK-C5227) immobilized on CM5 Chip can bind Human ANGPTL7 Protein, His Tag (Cat. No. AN7-H52H3) with an affinity constant of 0.792 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Leukocyte immunoglobulin-like receptor subfamily B member 4
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Phase 2 Clinical
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