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Product Details
Synonyms
CD112, Nectin-2, PVRL2
Source
Biotinylated Human Nectin-2, His,Avitag (CD2-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).
Predicted N-terminus: Gln 32
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 39.1 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human Nectin-2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) is more than 95% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human PVRIG, Fc Tag (Cat. No. PVG-H5257) with a linear range of 0.6-10 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) with a linear range of 0.039-0.625 μg/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
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