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Product Details
Synonyms
NECTIN4, LNIR, PRR4, PVRL4
Source
Human Nectin-4, His Tag (NE4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).
Predicted N-terminus: Gly 32
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.0 kDa. The protein migrates as 40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Nectin-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Nectin-4, His Tag (Cat. No. NE4-H52H3) is more than 95% and the molecular weight of this protein is around 30-46 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Nectin-4, His Tag (Cat. No. NE4-H52H3) at 1 μg/mL (100 μL/well) can bind Enfortumab with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Human Nectin-4, His Tag (Cat. No. NE4-H52H3) at 5 μg/mL (100 μL/well) can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with a linear range of 0.039-1.25 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Mouse Anti-Nectin-4 Antibody (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface can bind Human Nectin-4, His Tag (Cat. No. NE4-H52H3) with an affinity constant of 58.2 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) on Protein A Biosensor, can bind Human Nectin-4, His Tag (Cat. No. NE4-H52H3) with an affinity constant of 0.271 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human Nectin-4, His Tag (Cat. No. NE4-H52H3) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 0.24 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
1***82019-10-11
LikesI Purchased this antigen for monoclonal antibody generation and antibody screening. The purity has been test by SDS-PAGE. It has a single band and no heterologous protein. It was used for antibody screening, and the screened antibody has a good affinity with the antigen.
Background
Recent Advances
Drug Development Progress- English Name：
Nectin-4
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
28 Details
- Highest Development Stage：
Approved
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