Human Neprilysin / MME / CD10 Protein, Tag Free (active enzyme)

Synonyms

MME, CALLA, CD10, DKFZp686O16152, MGC126681, MGC126707, NEP, SFE, Neprilysin

Source

Human Neprilysin, Tag Free (MME-H521b) is expressed from human 293 cells (HEK293). It contains AA Tyr 52 - Trp 750 (Accession # NP_000893.2).

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries no "tag".

The protein has a calculated MW of 80.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process