Influenza A (A/Sydney/1304/2022) Neuraminidase (NA) Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
NE2-V5247-100ug
$360.00
NE2-V5247-1mg
$2760.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    Neuraminidase/NA (Influenza Virus)

  • Source

    Influenza A (A/Sydney/1304/2022) Neuraminidase (NA) Protein, His Tag (NE2-V5247) is expressed from human 293 cells (HEK293). It contains AA Lys 80- Thr 469 (Accession # EPI2304606, GISAID).

    Predicted N-terminus: His

    Request for sequence

  • Molecular Characterization

    Neuraminidase/NA (Influenza Virus) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Neuraminidase/NA (Influenza Virus) SDS-PAGE

    Influenza A (A/Sydney/1304/2022) Neuraminidase (NA) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Neuraminidase/NA (Influenza Virus) SEC-MALS

    The purity of Influenza A (A/Sydney/1304/2022) Neuraminidase (NA) Protein, His Tag (Cat. No. NE2-V5247) is more than 95% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Neuraminidase/NA (Influenza Virus) ELISA

    Immobilized Influenza A (A/Sydney/1304/2022) Neuraminidase (NA) Protein, His Tag (Cat. No. NE2-V5247) at 1 μg/mL (100 μL/well) can bind Anti-NA(A/PAN) Antibody,Mouse IgG with a linear range of 4-63 ng/mL (QC tested).

    Protocol

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Background

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

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