Product Details
Synonym
NRP1, Neuropilin-1, NRP, VEGF165R, CD304
Source
Biotinylated Human Neuropilin-1, His,Avitag (NR1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Lys 644 (Accession # AAH07533.1).
Predicted N-terminus: Phe 22
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 73.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Biotinylated Human Neuropilin-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA

Immobilized Human VEGF165, premium grade (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
Bioactivity-BLI

Loaded Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) on SA Biosensor, can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H3) with an affinity constant of 0.663 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
Neuropilin-1
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
3 Details
- Highest Development Stage:
Phase 2 Clinical
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