Human NFL Protein, His Tag (HPLC verified)

Customer Reviews
Cat. No. / Size
Price
Qty
NFL-H51H4-100ug
$380.00
NFL-H51H4-500ug (250ug X 2)
$1150.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Neurofilament light polypeptide

  • Source

    Human NFL Protein, His Tag (NFL-H51H4) is expressed from E. coli cells. It contains AA Ser 2 - Asp 543 (Accession # P07196-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    NFL Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 63.4 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • SDS-PAGE

    NFL SDS-PAGE

    Human NFL Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    NFL SEC-HPLC

    The purity of Human NFL Protein, His Tag (Cat. No. NFL-H51H4) was greater than 90% as determined by SEC-HPLC.

  • Bioactivity-ELISA

     NFL ELISA

    Immobilized Human NFL Protein, His Tag (Cat. No. NFL-H51H4) at 1 μg/mL (100 μL/well) can bind NFL Monoclonal Antibody with a linear range of 0.06-4 ng/mL (QC tested).

    Protocol

Customer Reviews
Writing Reviews

Background

Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronal cytoskeleton. Neurofilaments usually contain three intermediate filament proteins: NFL, NFM, and NFH which are involved in the maintenance of neuronal caliber. Neurofilament heavy (NFH) can server as biomarkers for several diseases by indicating axonal injury.

Recent Advances

 
Contact Us
Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
  • Background
Contact Us

[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521

[North America]: 1 Innovation Way, Newark, DE 19711, USA
[Switzerland]: ACRObiosystems AG, c/o Zug Dammstrasse Centre AG, Dammstrasse 19, 6300 Zug Switzerland