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Product Details
Synonyms
NKG2D, CD314, KLRK1, NK cell receptor D
Source
Biotinylated Human NKG2D Protein, Avitag,His Tag (NKD-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Ile 73 - Val 216 (Accession # P26718-1).
Predicted N-terminus: Gly
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 20.2 kDa. The protein migrates as 28-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human NKG2D Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human NKG2D Protein, Avitag,His Tag (Cat. No. NKD-H82Q3) is more than 85% and the molecular weight of this protein is around 36-46 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human MICA, Fc Tag (Cat. No. MIA-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human NKG2D Protein, Avitag,His Tag (Cat. No. NKD-H82Q3) with a linear range of 5-156 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human NKG2D Protein, Avitag,His Tag (Cat. No. NKD-H82Q3) at 1 μg/mL (100 μL/well) on streptavidin (STN-N5116) precoated (0.5 μg/well) plate can bind Human MICA, Fc Tag (Cat. No. MIA-H5253) with a linear range of 0.05-0.8 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
NKG2-D type II integral membrane protein
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
10 Details
- Highest Development Stage：
Phase 2 Clinical
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