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Product Details
Synonyms
Reticulon-4 receptor，Nogo-66 receptor
Source
Human Nogo Receptor, His Tag (NOR-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 27 - Ser 447 (Accession # Q9BZR6).
Predicted N-terminus: Cys 27
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 47.2 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Nogo Receptor, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-SPR
Human Nogo Receptor, His Tag (Cat. No. NOR-H52H3) capture on NTA-Series S sensor chip can bind Human MAG, Fc Tag (Cat. No. MAG-H5254) with an affinity constant of 2.21 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Human MAG, Fc Tag (Cat. No. MAG-H5254) captured on Protein A Chip can bind Human Nogo Receptor, His Tag (Cat. No. NOR-H52H3) with an affinity constant of 4.81 μM as determined in SPR assay (Biacore 8K) (Routinely tested).
Protocol
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