SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag

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Cat. No. / Size
Price
Qty
NS1-C51H7-100ug
$410.00
NS1-C51H7-1mg (200ug X 5)
$2945.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    NSP1, nsp1, Leader protein, Host translation inhibitor nsp1

  • Source

    SARS-CoV-2 NSP1, His Tag (NS1-C51H7) is expressed from E. coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    NSP1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    NSP1 SDS-PAGE

    SARS-CoV-2 NSP1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

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Background

The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.

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