Save Product
Product Details
Synonyms
HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor, NTF3, NT3.Neurotrophin3
Source
Human NT-3 Protein, Tag Free (NT3-H5213) is expressed from human 293 cells (HEK293). It contains AA Tyr 139 - Thr 257 (Accession # P20783).
Predicted N-terminus: Tyr 139
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 13.6 kDa. The protein migrates as 13-14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human NT-3 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human NT-3 Protein, Tag Free (Cat. No. NT3-H5213) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human NT-3 Protein, Tag Free (Cat. No. NT3-H5213) at 1 μg/mL (100 μL/well) can bind Human TrkC Protein, Fc Tag (Cat. No. TRC-H5256) with a linear range of 0.8-13 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Neurotrophin-3
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
